2018

Second Harmonic Generation Microscopy Provides Accurate Automated Staging of Liver Fibrosis in Patients with Non-alcoholic Fatty Liver Disease

Pik Eu Chang, George Boon Bee Goh, Wei Qiang Leow, Liang Shen, Kiat Hon Lim, Chee Kiat Tan. PLoS ONE 13(6):e0199166.https://doi.org/10.1371/journal.pone.0199166.

• Improved Performance of Quantitative Collagen Parameters versus Standard Histology in Longitudinal Assessment of Nonadvanced Liver Fibrosis for Chronic Hepatitis B

Y. Wang, X. Liang, J. Yang, H. Wang, D. Tan, S. Chen, J. Cheng, Y. Chen, J. Sun, F. Rong, W. Yang, H. Liu, Z. Liu, Y. Zheng, J. Liang, S. Li, Z. Liu, J. Hou. Journal of Viral Hepatitis, https://doi.org/10.1111/jvh.12835.

• Advanced Septa Size Quantitation Determines the Evaluation of Histological Fibrosis Outcome in Chronic Hepatitis B Patients

Bingqiong Wang, Yameng Sun, Jialing Zhou, Xiaoning Wu, Shuyan Chen, Shanshan Wu, Hui Liu, Tailing Wang, Xiaojuan Ou, Jidong Jia, Hong You. Modern Pathology, https://doi.org/10.1038/s41379-018-0059-x.

• Quantitative Assessment of Liver Fibrosis (qFibrosis) Reveals Precise Outcomes in Ishak "Stable" Patients on Anti-HBV Therapy

Yameng Sun, Jialing Zhou, Xiaoning Wu, Yongpeng Chen, Hongxin Piao, Lungen Lu, Huiguo Ding, Yuemin Nan, Wei Jiang, Tailing Wang, Hui Liu, Xiaojuan Ou, Aileen Wee, Neil D. Theise, Jidong Jia, Hong You. Nature.com, Scientific Reports. 2018 Feb 14;8(1):2989. doi: 10.1038/s41598-018-21179-2.

• INT-767 Improves Histopathological Features in a Diet-induced ob/ob Mouse Model of Biopsy-confirmed Non-alcoholic Steatohepatitis (NASH)

Jonathan D Roth, Michael Feigh, Sanne S Veidal, Louise K D Fensholdt, Kristoffer T G Rigbolt, Henrik H Hansen, Li C Chen, Mathieu Petitjean, Weslyn W Friley, Niels Vrang, Jacob Jelsing and Mark Young. World Journal of Gastroenterology. 2018 Jan 14;24(2):195-210. doi: 10.3748/wjg.v24.i2.195.

• Fully Automated Quantitative Assessment for NASH Using the Genesis®200 Imaging System

Stephen A. Harrison, MD, Zachary Goodman, MD, PhD, Katherine M. Cebe, MD, Lakshmi Alaparthi, Fanny Monge, Peter G. Traber, MD, Dean Tai, PhD. Presented at NASH-TAG 2018. 

2017

• Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease

Feng Liu, MD, Jing-Min Zhao, MD, Hui-Ying Rao, MD, Wei-Miao Yu, MD, Wei Zhang, MD, Neil D. Theise, MD, Aileen Wee, MBBS, FRCPath, FRCPA, and Lai Wei, MD. Am J Clin Pathol. 2017 Nov 20;148(6):502-512. doi: 10.1093/ajcp/aqx104 

• New Standards in Fibrosis Quantification: The Value of Label-free Second Harmonic Generation

Li Chen, Mathieu Petitjean at the Anti-fibrotic Drug Development Forum, November 13-14, 2017, Boston USA

• Detailed analysis of the antifibrotic effect of INT-767, a dual FXR/TGR5 agonist in an obese mouse model of diet-induced and biopsy-confirmed NASH

Sanne Skovgård Veidal, Michael Feigh, Henrik B Hansen, Jacob Jelsing, Niels Vrang, Mathieu Petitjean, Li Chen, Mark Young, Jonathan Roth. Presidential Award Poster. Presented at AASLD 2017, October 20-23, 2017.

• Application of Human NASH-based Transcriptome and Metabolome Profiles in Preclinical Models

Martine Morrison, TNO, Metabolic Health Research, The Netherlands. Presented at AASLD 2017.

• Quantitative Imaging and Deep Profiling of Collagen Structure for Asian Triple Negative Breast Cancer

Dr Yu Weimiao, presented at the 3rd Digital Pathology Congress Asia-Pacific 2017, Guangzhou, China.

• Multiphoton Imaging of Breast Cancer

Clinnovate Health UK, presented at the Glasgow Molecular Pathology Node Symposium 2017, University of Glasgow.

• Automated, Fully Quantitative Analysis by Using Second Harmonic Generation Microscopy in the Fibrosis Progression of Nonalcoholic Fatty Liver Disease

Feng Liu*, Jing-Min Zhao, Pei-Hao Liu, Hui-Ying Rao, Wei Zhang, Nan Wu, Lai Wei. Presented at EASL 2017.

• Development and Validation of an Automated Assessment of Liver Fibrosis Using SHG in Patients with NAFLD

Jason Chang , George Boon-Bee Goh, Wei Qiang Leow , Yayun Ren , Wei Keat Wan , Kiat Hon Lim , Chee Kiat Tan. Presented at EASL 2017.

• Novel Methods in Imaging and Quantification of Skeletal Muscle Fibrosis Associated with Severe Muscular Dystrophy

David W. Hammers, Coar C. Coker, Li Chen, Matt Petitjean, Elizabeth R. Barton, H.Lee Sweeney presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017.

• Use of Second Harmonic Generation (SHG) and 2-Photon Emission Imaging to Quantify and Describe the Structure of Tissue Fibrosis in the Rat TNBS Intestinal Fibrosis Model

Laura Johnson, Li.Chen, P. Higgings et all. Poster #2008 presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017.

• Concurrent Semi-Automated Quantification of Liver Fibrosis and Fat Using Label-free Second Harmonic Generation Imaging in Animal Models

M. Petitjean, J. Schwartz, Li Chen. Poster #3003 - presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017.

• Quantification of Septa in Liver Fibrosis Using Second Harmonic Generation Imaging

Li Chen, Mohammad Naser, et all. Poster #1016 - presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017.

• Comparative Efficacy of INT-767 Vs. Liraglutide in Gubra AMLN Mice

Jonathan Roth et All. Intercept Pharmaceuticals. Keystone Symposia Workshop: approaches to NASH Treatment, March 7th 2017. 

• New Classification of Liver Biopsy Assessment for Fibrosis in Chronic Hepatitis B Patients Before and After Treatment

Yameng Sun et all. Journal of Hepatology, doi: 10.1002/hep.29009.

2016

• Quantitative Imaging of Fibrotic and Morphological Changes in Liver of Nonalcoholic Steatohepatitis (NASH) Model Mice by Second Harmonic Generation (SHG) and Auto-Fluorescence (AF) Imaging Using Two-Photon Excitation Microscopy (TPEM)

Shin Yamamoto, Yusuke Oshima, Takashi Saitou, Takao Watanabe, Teruki Miyake, Osamu Yoshida, Yoshio Tokumoto, Masanori Abe, Bunzo Matsuura, Yoichi Hiasa, Takeshi Imamura. Biochemistry and Biophysics Reports 8 (2016) 277–283; https://doi.org/10.1016/j.bbrep.2016.09.010.

• Automated Evaluation of Liver Fibrosis in CCI4, BDL and TAA Rodent Models Using Second Harmonic Generation / Two-Photon Excited Fluorescence Microscopy

Feng Liu et al. Laboratory Investigation (2016) , 1-9. 2016 USCAP

• Quantitative Assessment for Fibrosis in NAFLD Using Collagen String Features in Specific Regions for Liver

Christopher J. Mulder, Zachary Goodman, Caroline Bolduc, Matthew F. Raines, Katherine M. Cebe, Lakshmi Alaparthi, Fanny Monge, Peter G. Traber, Dean Tai, Stephen A. Harrison. APASL 2016.

• Second Harmonic Generation Microscopy: A Novel Automated Method for Quantification of Hepatic Steatosis in Chronic Liver Disease

George B. Goh, Wei Qiang Leow, Shen Liang, Wei Keat Wan, Dean C. Tai, Kiat Hon Lim, Chee Kiat Tan, Jason P. Chang Poster # 1518 at AASLD 2016

• Phenotypical Characterization of Intrahepatic Cholangiocarcinomas Stroma: The More Fibrous, the Less Pejorative

L. Blaise, M. Albuquerque, C. Neuzillet, N. Guedj, J. Cros, V. Paradis, INSERM UMR1149 Beaujon Hospital, Clichy, France, Poster Presentation, AASLD Liver Meeting 2016

• Development and Validation of an Automated Optical Imaging System to Differentiate Severity of Hepatitis B- and Non-alcoholic Steatohepatitis-related Fibrosis in Patients with Concomitant Disease

Jason P. Chang, George B. Goh, Wei Qiang Leow, Wei Keat Wan, Dean C. Tai, Kiat Hon Lim, Chee Kiat Tan, Poster # 407 at AASLD 2016.

• Objective Quantitative Evaluation of Anti-fibrotic Agents in Animal Models

Kenneth Zhang, Li Chen, Bertha Xue, Chen Hu, Laurent Gole, and Chuan-Chu Chou. Poster #69 at The First International Conference on Tissue Repair, Regeneration , and Fibrosis, September 21-25, 2016.

• Evaluation of Liver Fibrosis: "Something Old, Something New..."

Zannis Almpanis, Maria Demonakou, Dina Tiniakos. Ann Gastroenterol 2016; 29 (4): 445-453. DOI: http://dx.doi.org/10.20524/aog.2016.0046. 

2015

• Automated Biphasic Morphological Assessment of Hepatitis B-related Liver Fibrosis Using Second Harmonic Generation Microscopy

Tong-Hong Wang, Tse-Ching Chen, Xiao Teng, Kung-Hao Liang & Chau-Ting Yeh. Scientific Reports, 5:12962; DOI: 10.1038/srep12962. 

• Low Cost and Compact Nonlinear (SHG/TPE) Laser Scanning Endoscope for Bio-medical Application

Liu, J., Lim, K. C., Li, H., Seck, H. L., Yu, X., Kok, S. W., et al. (2015). Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 9304, 93041K-.

• Multiphoton Microscopy: A Potential Intraoperative Tool for the Detection of Carcinoma In Situ in Human Bladder

Manu Jain, MD; Brian D. Robinson, MD; Maria M. Shevchuk, MD; Amit Aggarwal, PhD; Bekheit Salamoon, MBBS; Justin M. Dubin, BS; Douglas S. Scherr, MD; Sushmita Mukherjee, PhD, Ms. Arch Pathol Lab Med. 2015; 139:796-804.

• Evaluation of Area-based Collagen Scoring by Nonlinear Microscopy in Chronic Hepatitis C-induced Liver Fibrosis

David Sevrain, Matthieu Dubreuil, Grace Elizabeth Dolman, Abed Zaitoun, William Irving, Indra Neil Guha, Christophe Odin, and Yann Le Grand. Biomed. Opt. Express, 1209-1218 (2015)

• Digital Whole Slide Imaging and Two Photon Microscopy for Liver Fibrosis Scoring and Quantification

Jolanta Jedrzkiewicz, Mary P Bronner, Erinn Downs-Kelly, Jessica Kohan, Leslie Rowe, Dean Tai, Mohamed Salama. Modern Pathol. 2015; 28(2):167A

• Stain-free Detection of Bone Marrow Fibrosis Using Two Photon Excitation and Second Harmonic Generation Microscopy

Rowe L, Etman A, Kohan J, Prchal J, Ho G, Liu DQ, Salama M. Modern Pathol. 2015; 28(2):523A. 

• Current Strategies for Quantitating Fibrosis in Liver Biopsy

Wang, Yan & Hou, Jin-Lin. (2015). Chinese medical journal. 128. 252-8. 10.4103/0366-6999.149223.

2014

• Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer by Analysis of Second Harmonic Generation Polarization and Emission Directionalit

Tilbury K, Lien CH, Chen SJ, Campagnola PJ. Biophysical journal. 2014;106(2):354-65 

2011

• Minimally Invasive Multiphoton and Harmonic Generation Imaging of Extracellular Matrix Structures in Lung Airway and Related Diseases. Pulmonary Pharmacology & Therapeutics

Abraham T, Hirota JA, Wadsworth S, Knight DA. 2011;24(5):407-96

• Aligned Collagen is a Prognostic Signature for Survival in Human Breast Carcinoma

Conklin MW, Eickhoff JC, Riching KM, et al. The American journal of pathology. 2011;178(3):1221-32 

2009

• Fibro-C-index: Comprehensive, Morphology-based Quantification of Liver Fibrosis Using Second Harmonic Generation and Two-Photon Microscopy

Tai DC, Tan N, Xu S, et al. Journal of biomedical optics. 2009; 14(4):044013

• Nonlinear Optical Microscopy: Use of Second Harmonic Generation and Two-Photon Microscopy for Automated Quantitative Liver Fibrosis Studies

Sun W, Chang S, Tai DC, et al. Journal of biomedical optics. 2008;13(6):064010 

2008

• Second Harmonic Microscopy to Quantify Renal Interstitial Fibrosis and Arterial Remodeling

Strupler, M., Hernest, M., Fligny, C., Martin, J. L., Tharaux, P. L., and Schanne-Klein, M. C. (2008), Journal of biomedical optics 13, 054041