Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application

Jia Yun Liu; Ken Choong Lim; Hao Li; Hon Luen Seck; Xia Yu; Shaw Wei Kok; Ying Zhang

doi:10.1117/12.2078809

2 March 2015 Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application

Jia Yun Liu, Ken Choong Lim, Hao Li, Hon Luen Seck, Xia Yu, Shaw Wei Kok, Ying Zhang

Proceedings Volume 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II; 93041K (2015) https://doi.org/10.1117/12.2078809
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Two-photon fluorescence (TPE) and second harmonic generation (SHG) can been used to extract biological information from tissues at the molecular level, which is blind to traditional microscopes. Through these two image contrast mechanisms, a nonlinear laser scanning endoscope (NLSE) is able to image tissue cells and the extra cellular matrix (ECM) through a special fiber and miniaturized scanner without the requirement of poisonous chemical staining. Therefore, NLSE reserves high potential for in-vivo pathological study and disease diagnosis. However, the high cost and bulky size of a NLSE system has become one of the major issues preventing this technology from practical clinical operation. In this paper, we report a fiber laser based multi-modality NLSE system with compact size and low cost, ideal for in-vivo applications in clinical environments. The demonstration of the developed NLSE nonlinear imaging capability on different bio-structures in liver, retina and skin are also presented.

Citation Download Citation

Jia Yun Liu, Ken Choong Lim, Hao Li, Hon Luen Seck, Xia Yu, Shaw Wei Kok, and Ying Zhang "Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application", Proc. SPIE 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II, 93041K (2 March 2015); https://doi.org/10.1117/12.2078809

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