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MPEF Microscopy Shines Best on Living Samples

MPEF microscopy, along with other nonlinear methods that include coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and second harmonic generation (SHG), is suitable for imaging whole cells — especially cells within intact tissue samples. While many fluorescence microscopy schemes use filtered lamps, LEDs and lasers as light sources that drive excitation-produced visualizations, MPEF methods require an ultrafast laser source that is focused onto the sample’s plane of observation. Typically, it also uses a combination of dichroic mirrors and longpass filters to filter the emitted light to the wavelengths of interest.

We found this article, which was published on BioPhotonics in October 2015, very insightful. View here for the full article.