Posters and Presentations


13 November 2017

Anti-Fibrotic Drug Development (AFDD) Summit 2017

New Standards in Fibrosis Quantification: the Value of Label-free Second Harmonic Generation

By Dr Mathieu Petitjean, CEO, MedNest, USA


20 October 2017

AASLD Liver Meeting 2017

At the AASLD Liver Meeting this year, the following presentations were conducted by our distinguished partner and collaborator respectively:


Detailed Analysis of the Antifibrotic Effect of INT-767, a Dual FXR/TGR5 Agonist in an Obese Mouse Model of Diet-induced and Biopsy-confirmed NASH

By Dr Mathieu Petitjean, CEO, MedNest, USA

The farnesoid X receptor (FXR) and G protein coupled receptor (GPCR) TGR5 have been implicated in the regulation of metabolism, inflammation and fibrosis in non-alcoholic steatohepatitis (NASH). A dual FXR/TGR5 agonist, INT-767, has been developed and is in early clinical development. Here we evaluated the efficacy of INT-767 on qualitative and qualitative histological parameters in an obese mouse model of diet-induced and biopsy-confirmed NASH. 

Application of Human NASH-based Transcriptome and Metabolome Profiles in Preclinical Models

By Dr Martine Morrison,  TNO, Metabolic Health Research, The Netherlands

Dr. Morrison presented on the representation of human-based molecular disease profiles in an established pre-clinical model for NASH research - the  Ldlr-/-.Leiden mouse – and the translational value of this model for the study of NASH-associated hepatic fibrosis.



17 September 2017

3rd Digital Pathology Congress Asia-Pacific 2017

At the 3rd Digital Pathology Congress Asia-Pacific 2017, Dr Yu Weimiao (Head, Computational BioImage Analysis (CBA) Unit, Institute of Molecular and Cell Biology, A*STAR) gave a presentation on Quantitative Imaging and Deep Profiling of Collagen Structure for Asian Triple Negative Breast Cancer: Novel perspectives for Breast Cancer Micro-Environment.

The ExtraCellular Matrix (ECM) maintains normal tissue polarity and architecture and also prevents cancer cell invasion. Therefore, deregulation and remodeling of the ECM dynamics are one of the prerequisite and hallmarks of cancer. The importance of stromal biology in breast tumor progression is highlighted in many studies.
We used a novel imaging solution to explore the collagen structure in TNBC: Second Harmonic Generation (SHG) and Two-Photon Emission (TPE) imaging: The SHG imaging will fully quantify the micro-environment, including collagen type I & III, blood vessel (angiogenesis) and stromal structures.
We developed an automatic image analysis solution to extract different features in the SHG images, including collagen structural information, which plays a key role in the aggressiveness of the breast cancer. The extracted parameters are then correlated with our clinical outcome.
Several imaging parameters from SHG-TPE technology have shown potential to clinicopathological parameters, such as tumor grade, size, lymph node staging, survival rate, as well as PD-L1/PD-1. Our deep profiling of the 388 patient samples shows novel understanding of the micro-environment change during the breast cancer progress.


28 November 2016

AASLD: The Liver Meeting 2016

HistoIndex was involved in contributing to the following posters:


1. Development and validation of an automated optical imaging system to differentiate severity of Hepatitis B- and Non-alcoholic steatohepatitis-related fibrosis in patients with concomitant disease

2. Phenotypical characterization of intrahepatic cholangiocarcinomas stroma : the more fibrous, the less pejorative

3. Second Harmonic Generation (SHG) microscopy : a novel automated method for quantification of hepatic steatosis in chronic liver disease


30 September 2016

BioImage Informatics Conference 2016

HistoIndex will also be giving a LunchTalk on 10th October on "HistoIndex Stain-Free Multiphoton Digital Pathology".

Click here for more information.


26 July 2016

14th Urological Association of Asia Congress 2016

The abstract for the following was presented at the abovementioned event:

“Predicting Aggressiveness in very Low Risk Asian Prostate Cancer on Biopsy with a Novel Multiphoton Microscopy Imaging Tool (HistoIndex®).

R Tiwari1, Yu WM2, Huang HH1, Tan PH3, H Ho1

1 Department of Urology, Singapore General Hospital; 2 Agency for Science, Technology and Research, Singapore; 3 Department of Pathology, Singapore General Hospital


20 February 2016

The Asian Pacific Association for the Study of the Liver (APASL) 2016

HistoIndex was involved in contributing to the following poster:


1Quantitative Assessment For Fibrosis In NAFLD Using Collagen String Features In Specific Regions For Liver

*Dr. Dean Tai, Chief Scientific Officer of HistoIndex, is one of the authors.


11 January 2016

Three Poster Presentations at the AASLD: The Liver Meeting 2015


782 qFibrosis scoring for different animal models with liver fibrosis induced by TAA, CCl4 or BDL

Feng Liu, Long Chen, Huiying Rao, Wei Zhang, Nan Wu, Lai Wei

Peking University People’s Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases


1436 Collagen scoring by qFibrosis in paired liver biopsies from chronic hepatitis C patients before and after treatment

Huiying Rao1, Feng Liu1, Aileen -. Wee2, Bo Feng1, Ming Yang1, Lai Wei1

1Peking University People’s Hospital, Peking University Hepatology Institute, 2Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore


1449 Second harmonic generation microscopy based quantitative assessment for HBV-associated liver fibrosis

Kai-Wen Huang

National Taiwan University Hospital


12 August 2015

Latest Paper of Histoindex's Genesis is published in Scientific Reports

Abstract: Liver fibrosis assessment by biopsy and conventional staining scores is based on histopathological criteria. Variations in sample preparation and the use of semi-quantitative histopathological methods commonly result in discrepancies between medical centers. Thus, minor changes in liver fibrosis might be overlooked in multi-center clinical trials, leading to statistically non-significant data. Here, we developed a computer-assisted, fully automated, staining-free method for hepatitis B-related liver fibrosis assessment. In total, 175 liver biopsies were divided into training (n = 105) and verification (n = 70) cohorts.

Click here for the full report.


11 August 2015

Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application

Abstract: Two-photon fluorescence (TPE) and second harmonic generation (SHG) can be used to extract biological information from tissues at the molecular level, which is blind to traditional microscopes. Through these two image contrast mechanisms, a nonlinear laser scanning endoscope (NLSE) is able to image tissue cells and the extra cellular matrix (ECM) through a special fiber and miniaturized scanner without the requirement of poisonous chemical staining.

Therefore, NLSE reserves high potential for in-vivo pathological study and disease diagnosis. However, the high cost and bulky size of a NLSE system has become one of the major issues preventing this technology from practical clinical operation. In this paper, we report a fiber laser based multi-modality NLSE system with compact size and low cost, ideal for in-vivo applications in clinical environments. The demonstration of the developed NLSE nonlinear imaging capability on different bio-structures in liver, retina and skin are also presented.

Keywords: Second-harmonic generation, Two-photon fluorescence, Ultrafast fiber laser, Endoscopy

Click here for the full report.


26 May 2015

Digestive Diseases Week 2015 in USA

We had the honour of having Prof Tan Chee Kiat and Dr. Jason Chang Pik Eu from the Department of Gastroenterology and Hepatology, Singapore General Hospital, to present a paper on HistoIndex's core technology at the abovementioned Conference.

This paper was titled, "Second harmonic generation microscopy - a novel tissue imaging technology for the differentiation and quantification of hepatitis B versus non-alcoholic steatohepatitis (NASH)-induced liver fibrosis".


19 May 2015

Chinese Medical Journal - Review Article

A review article published in the Chinese Medical Journal.

Click here to view full article.


28 March 2015

2015 Annual USCAP Meeting in USA

The following two papers were presented at this meeting:

(a)  "Digital whole slide imaging and two photon microscopy for Liver fibrosis scoring and quantification", and;

(b)  "Stain-free detection of bone marrow fibrosis using two photon excitation and second harmonic generation microscopy"


23 March 2015

Laennec Liver Pathology Society Annual Meeting Paris 2015

HistoIndex will be participating in this annual Laennec meeting in Paris. Both Dr. Dean Tai, Chief Scientific Officer of HistoIndex and Dr. Katarzyna Zarychta, Vice President of Sales in Europe & Middle East will be attending this conference.


16 March 2015

24th Conference of APASL 2015, Istanbul

We had the honour of having Dr. Huang Kai-Wen, Attending Physician and Assistant Professor of the Department of Surgery and Hepatitis Research Center at the National Taiwan University Hospital, to present a paper on HistoIndex's core technology at this conference.

The paper presented was titled “The Automatic Measurement System of Liver Fibrosis Status by Second-Harmonic Generation Microscopy”.